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. 2016 Nov 17;7:1838. doi: 10.3389/fmicb.2016.01838

FIGURE 1.

FIGURE 1

Effect of Paraburkholderia phytofirmans PsJN-derived mutants on growth stimulation of Arabidopsis thaliana plants. Root growth, rosette area and fresh weight of A. thaliana col-0 grown in gnotobiotic in vitro cultures using half strength MS agar medium inoculated with 1 × 104 CFU/ml of P. phytofirmans (PsJN), a heat-killed PsJN inoculum (HK-PsJN), the PsJN-derived mutants (acdS, iacC, bpI.1, or fliA), or non-inoculated medium (N. I.). Plants were transferred at 11 days after sowing (DAS) to fresh MS1/2 agar with no added NaCl or CaCl2 (untreated controls) (A), or MS1/2 agar with 150/15 mM NaCl/CaCl2 (B). Growth parameters were registered at 21 DAS. Bars show mean percentage values relative to N. I. plants, and the error bars indicate standard deviations from experiments with 30 plants analyzed for each bacterium and salt treatment. Different letters indicate statistically significant differences among bacterial treatments within each salt concentration for each measured parameter (One way ANOVA Tukey’s HSD tests; p < 0.05).