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. 2016 Nov 17;7:1677. doi: 10.3389/fpls.2016.01677

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Copyright © 2016 Wei, Wang, Chang, Wu, Liu, Meng and Zou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Strategy used to generate a population of new-type B. juncea. The Brassica allohexaploid in which the A^r and B^c subgenomes from B. carinata (B^cB^cC^cC^c) and B. rapa (A^rA^r) (Tian et al., 2010) were combined was used as the donor of exotic subgenomes. The first step in the current study involved interspecific crosses between various hexaploid and traditional B. juncea to transfer the A^r and B^c subgenomes to B. juncea. Successive phenotypic and cytological selection was then conducted up to the F₆ generation, whereas genomic surveying was mainly conducted at the F₃ generation.