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. 2016 Nov 3;2016:3128235. doi: 10.1155/2016/3128235

Figure 1.

Figure 1

Effect of THSG on the luciferase reporter activity of the klotho gene. HEK-293 cells that were transiently transfected with luciferase reporter plasmids driven by a human klotho promoter (from −1000 to +299) and reporter pRL-SV40 plasmid containing the Renilla luciferase gene as normalization were stimulated with the indicated doses of THSG for 16 h. Cells were lysed and analyzed for luciferase activity (n = 3). Data are expressed as mean ± SD. ∗ P < 0.05 versus control empty plasmid, and ∗∗ P < 0.01 versus control empty plasmid.