Table 1.

Primer sequences of target genes and annealing temperature.

Gene	Primer sequences	Annealing temperature
Klotho (138 bp)	Sense: 5′-ACTTTCTTCTGCCCTATTTCACG-3′	58°C
	Antisense: 5′-CCAGGTAATCGTTGTATTTTATCGG-3′
GAPDH (192 bp)	Sense: 5′-GATCCCGCTAACATCAAATG-3′	58°C
	Antisense: 5′-GAGGGAGTTGTCATATTTCTC-3′