Table 1.

Genes required for FA tolerance and their associated GO or MIPS categories.

GO Biological Process	p-value	Genes identified	ka	fb
Response to virus [GO:0009615]	1.31E-006	SKI8, SLH1, SKI2, SKI3	4	4
Nuclear-transcribed mRNA catabolic process, exonucleolytic, 3′–5′ [GO:0034427]	7.36E-004	SKI8, SKI2, SKI7, SKI3	4	13
DNA repair [GO:0006281]	1.38E-003	NTG1, SAW1, RAD18, RAD59, RAD57, MUS81, RAD27, APN1, TRM2, RAD5, MEC3, SGS1, DNL4, HNT3, PHR1	15	183
Nuclear-transcribed mRNA catabolic process, 3′-5′ exonucleolytic nonsense-mediated decay [GO:0070478]	2.20E-003	SKI8, SKI2, SKI7, SKI3	4	17
Response to DNA damage stimulus [GO:0006974]	2.85E-003	NTG1, SAW1, RAD18, RAD59, RAD57, MUS81, RAD27, APN1, RAD5, MEC3, YIM1, SGS1, DNL4, HNT3, PHR1	15	197
Nucleus organization [GO:0006997]	3.39E-003	TOM1, GSP2	2	3
Cellular monovalent inorganic cation homeostasis [GO:0030004]	3.39E-003	NHX1, VHS3	2	3
Telomere maintenance via recombination [GO:0000722]	3.40E-003	RAD59, RAD57, MEC3, SGS1	4	19
DNA recombination [GO:0006310]	3.50E-003	RAD59, MUS81, SHU1, CDC73, SGS1, DNL4	6	44
Cell redox homeostasis [GO:0045454]	3.62E-003	GRX6, TRX2, DOT5, POR2, AHP1	5	31
Nucleotide metabolic process [GO:0009117]	5.26E-003	APA1, APA2, AMD1	3	11
Response to singlet oxygen [GO:0000304]	6.63E-003	SNQ2, SKN7	2	4
Osmosensory signaling pathway via two-component system [GO:0007234]	6.63E-003	SSK1, SSK2	2	4
Nuclear-transcribed mRNA catabolic process, non-stop decay [GO:0070481]	6.98E-003	SKI8, SKI2, SKI7, SKI3	4	23
Cellular response to oxidative stress [GO:0034599]	7.43E-003	NTG1, GRX6, TRX2, GRE3, DOT5, TMA19, AHP1	7	67
CVT pathway [GO:0032258]	7.87E-003	COG7, COG8, COG6, COG5, VPS30	5	37
Nucleocytoplasmic transport [GO:0006913]	7.87E-003	TOM1, NUP100, NUP188, NUP53, GSP2	5	37
Intra-Golgi vesicle-mediated transport [GO:0006891]	8.16E-003	COG7, COG8, COG6, COG5	4	24
DNA metabolic process [GO:0006259]	8.67E-003	RAD57, MUS81, MEC3	3	13
Postreplication repair [GO:0006301]	8.67E-003	PAN2, POL32, RAD5	3	13
Response to drug [GO:0042493]	9.45E-003	SNQ2, YKL075C, TDA5, IRC21	4	25
GO cellular component	p-value	Genes identified	ka	fb
Ski complex [GO:0055087]	1.31E-006	SKI8, SKI2, SKI7, SKI3	4	4
Golgi transport complex [GO:0017119]	8.25E-005	COG7, COG8, COG6, COG5	4	8
Phosphopantothenoylcysteine decarboxylase complex [GO:0071513]	3.39E-003	SIS2, VHS3	2	3
Polysomal ribosome [GO:0042788]	6.63E-003	SLH1, TMA46	2	4
MIPS functional classification	p-value	Genes identified	ka	fb
DNA repair [10.01.05.01]	9.28E-005	NTG1, RAD18, RAD59, RAD57, MUS81, ECM32, PAN2, POL32, RAD27, APN1, DOA1, RAD5, MEC3, DNL4, ULS1, PHR1	16	159
Oxygen and radical detoxification [32.07.07]	5.24E-004	TRX2, DOT5, SSK1, AHP1	4	12
Electromagnetic waves stress response (e.g. UV, X-ray) [32.01.13]	1.16E-003	RAD61, PHR1	2	2
Detoxification by export [32.07.05]	3.39E-003	QDR2, YRM1	2	3
osmosensing and response [34.11.03.13]	6.19E-003	SLT2, PBS2, SIS2, SSK1, SSK2	5	35
RNA transport [20.01.21]	8.66E-003	GBP2, NUP100, SRN2, NUP188, NUP53, TPM1, TEX1, GSP2	8	86

Strains identified as sensitive to FA by DSSA (n = 225) were input into FunSpec and analyzed for overrepresented biological attributes.

^aNumber of genes from category identified as sensitive to FA.

^bTotal number of genes in GO or MIPS category.