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. 2016 Nov 2;4(21):e12944. doi: 10.14814/phy2.12944

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© 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Additional exogenous fatty acid treatment (45 μmol/L oleic acid, 30 μmol/L palmitic acid, and 25 μmol/L linoleic acid), does not significantly change intracellular TG and media 3OHB levels in HepG2 cells after 7 days of DMSO treatment (A and B). Furthermore, exogenous fatty acid treatment has no additional effect in the mRNA expression of FAS and ACC1 after 7 days of DMSO treatment (C and D). Data presented are mean ± SD of n = 3 experiments. The relative expression ratio was calculated using the following: Ratio = ΔCt_[target]/ΔCt_[ref] (Pfaffl 2001) and expression values were normalized to 18 sec.