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. 2016 Nov 8;4(21):e13016. doi: 10.14814/phy2.13016

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© 2016 EA Pharma Co., Ltd. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Alanine aminotransferase (ALT; hepatitis), hepatic triglycerides (TG; fat accumulation in the liver), hepatic cholesterol (TCHO), and gene expression levels in the livers of mice fed with steatohepatitis‐inducing high‐fat diet (STHD‐01). ALT (A), hepatic TG (B), and hepatic TCHO (C) levels, as well as the expression levels of Mcp1 (D), Tnf (E), and Cd68 (F) in the liver were determined after feeding with standard diet (SD) or STHD‐01 for 1, 3, 6, and 10 days. At each time point, n = 4–5 in each group (SD and STHD‐01. Bars represent the means ± standard errors. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the SD group (at each time point, Student's t‐test).