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. 2016 Nov 8;4(21):e13016. doi: 10.14814/phy2.13016

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© 2016 EA Pharma Co., Ltd. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effect of branched‐chain amino acids (BCAAs) on fibrosis in mice fed with steatohepatitis‐inducing high‐fat diet (STHD‐01) or standard diet (SD) for 30 weeks after diethylnitrosamine (DEN) pretreatment. The percentage of Sirius red‐positive areas indicating liver fibrosis (A, n = 9) and score for tumor size and number (B). Comparison between DEN/SD+3% casein and DEN/STHD‐01 + 3% casein, using the Student's t‐test (***P < 0.001). Comparison between DEN/STHD‐01 + 3% casein and DEN/STHD‐01 + 3% BCAA using the Student's t‐test (^## P < 0.01).