Table 2.
Changes in gene expression levels in the livers of mice fed with a steatohepatitis‐inducing high‐fat diet (STHD‐01) for 36 weeks, or for 30 weeks after diethylnitrosamine (DEN) pretreatment
| Gene name | 36‐week feeding | 30‐week feeding after DEN pretreatment | ||
|---|---|---|---|---|
| STHD‐01/SD | P‐value | STHD‐01/SD | P‐value | |
| Inflammation | ||||
| Tnf | 5.8 | 0.0008 | 8.0 | 0.0001 |
| Mcp1 | 8.2 | 0.0009 | 7.2 | 0.0005 |
| Il6 | 2.2 | 0.0001 | 3.3 | 0.0009 |
| Spp1 (osteopontin) | 9.3 | 0.0006 | 12.1 | 0.0003 |
| Adgre1 (F4/80) | 2.8 | 0.0001 | 2.6 | 0.0006 |
| Il17 | 1.4 | 0.0386 | 2.9 | 0.0020 |
| Nos2 | 6.8 | 0.0020 | 12.9 | 0.0002 |
| Fibrosis | ||||
| Col1a1 | 27.4 | 0.0001 | 15.2 | 0.0001 |
| Acta2 (alpha SMA) | 4.7 | 0.0004 | 3.3 | 0.0002 |
| Serpinh1 (HSP47) | 4.2 | 0.0002 | 4.8 | 0.0001 |
| Timp1 | 27.4 | 0.0001 | 24.7 | 0.0001 |
| Tgfb1 | 2.3 | 0.0001 | 2.5 | 0.0002 |
| Premalignant lesions | ||||
| Epcam | 14.1 | 0.0013 | 18.5 | 0.0008 |
| Krt8 (Cytokeratin8) | 4.4 | 0.0001 | 3.9 | 0.0003 |
| Ctnnb1 (beta‐Catenin) | 1.5 | 0.0001 | 1.8 | 0.0011 |
| Axin2 (conductin) | 2.3 | 0.0367 | 2.9 | 0.0032 |
| Oxidative stress | ||||
| Nrf2 | 1.7 | 0.0000 | 4.8 | 0.0001 |
| Hmox1 | 1.8 | 0.0002 | 2.5 | 0.0187 |
| Endoplasmic reticulum stress | ||||
| Atf4 | 1.8 | 0.0393 | 1.7 | 0.0033 |
| Eif2ak3(Perk) | 2.7 | 0.0001 | 4.8 | 0.0005 |
| Hspa5 (Grp78) | 1.3 | 0.0598 | 1.6 | 0.0333 |
| Ddit3 (chop) | 1.9 | 0.0042 | 3.4 | 0.0001 |
| Xbp1 | 1.3 | 0.0435 | 1.6 | 0.0033 |
| Traf2 | 2.2 | 0.0001 | 2.4 | 0.0001 |
| Metabolism | ||||
| Cyp7a1 | 1.5 | 0.1650 | 3.9 | 0.0001 |
| Phgdh | 1.9 | 0.0251 | 3.3 | 0.0026 |
| Pparg | 2.6 | 0.0000 | 4.2 | 0.0001 |
| Mthfd2 | 4.6 | 0.0033 | 4.5 | 0.0003 |
| Ucp2 | 3.7 | 0.0001 | 4.0 | 0.0001 |
| Fgf21 | 5.8 | 0.0948 | 4.3 | 0.0286 |
| Other genes | ||||
| Trp73 (p73) | 1.7 | 0.1805 | 4.5 | 0.0252 |
| Sema7a | 2.8 | 0.0016 | 5.6 | 0.0006 |
| Nfatc1 (NFAT2) | 1.6 | 0.0032 | 2.2 | 0.0001 |
| Itgb1 (integrin beta 1) | 1.6 | 0.0000 | 1.6 | 0.0026 |
| Tpt1 (p21) | 1.9 | 0.0029 | 2.5 | 0.0001 |
For each gene, the values represent the ratio between the expression level in the STHD‐01 group and that in the standard diet (SD) group (all gene expression levels are normalized to Gapdh expression). The P‐values are derived from t‐tests of the SD versus the STHD‐01 groups. See Table S1 for a description of the primers used for analyzing the expression of each gene.