Figure 6.

Airway draining lymph node CD4⁺ T‐cell subset percentages and numbers in OVA‐sensitized and challenged BALB/c WT and CD103^−/− mice. Adult BALB/c WT and CD103 KO mice were sensitized and challenged with OVA or saline as described for Figure 1. At 24 h after final aerosol, ADLN were harvested, and single cell suspensions prepared and analyzed by flow cytometry for total CD4⁺ T cells, CD4⁺ FoxP3⁺ CD25⁺ T_reg and CD4⁺ FoxP3^‐ CD25⁺ T_eff. (A) Percentages and (B) total numbers of total, T_reg and T_eff CD4⁺ T cells in ADLN of saline‐ or OVA‐challenged WT or CD103 KO mice. (data are three independent experiments with n = 5 mice/group in pooled samples; means +/− SEM). *P < 0.05 by one‐tailed Mann–Whitney U‐Test. ADLN, airway draining lymph node; WT, wild type; KO, knockout; OVA, ovalbumin.