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. 2016 Nov 17;6:37251. doi: 10.1038/srep37251

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A–D) Typical examples of the induction of c-Fos protein in the spinal dorsal horn induced by bee venom injection in vehicle-treated mice (A) and in groups treated with different doses of GAS (B–D). (E) Schematic diagram showing the counting of c-Fos-positive cells in different layers of the spinal dorsal horn. (F,G) Quantitative summary showing the inhibition of c-Fos expression in both the superficial (F) and deep layer of the spinal dorsal horn by GAS in a dose-dependent manner (n = 3, P < 0.05). All data are represented as mean ± S.E.M. Scale bar represents 100 μm in (A–E). *P < 0.05.