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. 2016 Nov 17;6:37303. doi: 10.1038/srep37303

Figure 2. Fluorescence analysis of mBBr to examine voltage-dependent conformational changes of VSD.

Figure 2

(a) The location of V119, which was mutated to Cys with the mBBr modification. (b–d) Fluorescence time courses of mBBr attached VSD on the liposomes with three different membrane potentials: (b) 0 mV, (c) −90 mV, and (d) −147 mV. (e) Relationship between the estimated membrane potential by di-4-ANEPPS analysis and the fluorescence intensity reduction ratio of mBBr at 100 s (upon the formation of the membrane potential).