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. 2016 Nov 17;6:37303. doi: 10.1038/srep37303

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(a) Schematic diagram of the intramolecular SS-bond detection. The SH form of the VSD mutant is PEGylated and exhibited a significant mobility shift (left), while the SS form of the VSD mutant is not PEGylated, leaving the mobility unchanged (right). (b) SDS-PAGE mobility of the VSD double Cys mutant (V42C/I130C) under reductive or oxidative conditions in the presence or absence of mal-PEG.