Skip to main content
. 2016 Nov 17;6:37339. doi: 10.1038/srep37339

Figure 1. Plasmid location of cmpg5300.05_29.

Figure 1

(a) Schematic illustration of the genetic organization of cmpg5300.05_29 on the putative plasmid contig 54. The primer pair PRO 7189 and PRO 7037 (black arrows) was used for constructing the probe for Southern hybridization. (b) Southern blot analysis. DNA gel profile for CMPG5300- Lane Gene ruler: High range ladder; Lane gDNA: Total genomic DNA preparation (band encircled in black); Lane pDNA undigested: Total plasmid DNA preparation-undigested (500 ng/ml); Lane pDNA digested: Total plasmid DNA digested with restriction enzymes (500 ng/ml); Lane DIG ladder: DIG ladder. DNA gel profile for WCFS1- Lane Gene ruler: High range ladder; Lane gDNA: Total genomic DNA; Lane DIG ladder: DIG ladder. (c) Southern blot of CMPG5300- Lane DIG ladder: DIG ladder; Lane pDNA digested: Total plasmid DNA digested (500 ng/ml); Lane pDNA undigested: Total plasmid DNA-undigested (500 ng/ml); Lane gDNA: Total genomic DNA. Southern blot of WCFS1- Lane gDNA: Total genomic DNA; Lane DIG ladder: DIG ladder. Southern hybridization of a 487-bp cmpg5300.05_28-specific labeled fragment gave a binding signal (at a size slightly higher than 21.2 kbp) marked in black solid arrow (in both DNA gel and Southern blot pictures) with the plasmid DNA preparations indicating the plasmid location of cmpg5300.05_29. No signal was obtained at the position of the total DNA (encircled). On the other hand, the 772-bp labeled fragment of L. plantarum WCFS1 (used as control) yielded a signal corresponding to band of the chromosomal DNA preparation.