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. 2016 Nov 17;6:37339. doi: 10.1038/srep37339

Figure 6. Activity of the lectin domain of Cmpg5300.05_29 against E. coli UTI89, S. aureus SH1000 and Rosenbach.

Figure 6

(a) Anti-biofilm activity of the lectin domain of Cmpg5300.05_29 against E. coli UTI89 expressed as percentage inhibition of biofilm formation. The purified lectin domain of Cmpg5300.05_29 was added after 0, 1.5 and 24 h to the biofilms at a concentration of 50 μg/ml and 200 μg/ml. (b) Growth of E. coli UTI89 in TSB in the presence of lectin domain of Cmpg5300.05_29 added at 50 μg/ml and 200 μg/ml. (c) Microscopic images of E. coli UTI89 biofilms grown only in 1/20 TSB and (d) with 50 μg/ml of FITC-labeled lectin domain of Cmpg5300.05_29. Holes in the biofilms are indicated with arrows. (e) Anti-biofilm activity of the lectin domain of Cmpg5300.05_29 against S. aureus SH1000 and Rosenbach. The purified lectin domain of Cmpg5300.05_29 was added after 0, 1.5 and 24 h to the biofilms at concentration of 50 μg/ml and 200 μg/ml. (f) Growth of S. aureus SH1000 and Rosenbach in TSB in presence of lectin domain of Cmpg5300.05_29 added at 50 μg/ml and 200 μg/ml. The dataset comparisons (biofilm formation with addition of lectin to biofilm without adding lectins) are considered significant (p < 0.05 indicated with one asterisks, p < 0.01 indicated with two asterisks and p < 0.001 indicated with three asterisks in the figure).