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. 2016 Nov 17;6:37270. doi: 10.1038/srep37270

Figure 4. Effect of CD133+ cells on fibrosis and vascularization at day 60.

Figure 4

(A) Plasma analysis for BUN and creatinine in AKI mice showing normalized renal function at day 60 after injury. (B) Representative micrographs of H&E and Masson’s trichromic staining of renal tissue in mice treated with PBS or CD133+ cells at day 60 after glycerol injection. Original magnification 200×. (C) qPCR analysis of whole kidney lysates for the pro-angiogenic factor VEGF and for the fibrotic factors TGF-β and α-SMA. Data are mean ± SD of three independent experiments and are normalized to GAPDH mRNA and to 1 for normal and expressed as relative quantification (RQ). (D) Quantification of fibrosis in the interstitium by Image J software was performed on 10 fields/section in two sections/mouse. T-test: **p < 0.01; * < 0.05.