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. 2016 Nov 17;6:37270. doi: 10.1038/srep37270

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Copyright © 2016, The Author(s)

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(A) Micrographs showing Western Blot analysis of hEPO in the supernatant cultures of CD133⁺ cells as compared to fibroblasts (3 representative cell lines). Recombinant EPO (rEPO) was loaded as control. (B) Quantitative RT-PCR analysis showing increased mRNA levels of human EPO and decreased GATA2 expression in CD133⁺ cells as compared to fibroblasts. Data are the mean ± SD of three experiments. Data are normalized to GAPDH mRNA and to 1 for CD133⁺ cells and expressed as relative quantification (RQ). (C) ChIP analysis of binding of GATA2 at EPO transcriptional motif in CD133⁺ cells in normoxia or hypoxia. Hypoxia reduced the binding of GATA2 by five folds in CD133⁺ cells. All data are means ± SD of 3 different experiments. T-test: *p < 0.01.