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. 2016 Nov 17;6:37255. doi: 10.1038/srep37255

Figure 2. Pan-neural knockdown of ap promotes arousal under LD cycles.

Figure 2

We generated transgenic flies (UAS-ap RNAi Pdf-GAL80) with both the UAS-ap RNAi and Pdf-GAL80 constructs in the second chromosome. Black circles and bars, nSyb-GAL4/+; gray circles and bars, UAS-ap RNAi Pdf-GAL80/+; green circles and bars, nSyb-GAL4/UAS-ap RNAi; orange circles and bars, nSyb-GAL4/UAS-ap RNAi Pdf-GAL80. All sleep/wake parameters (daily sleep pattern, total sleep amount, sleep-bout duration, wake-bout duration, waking time, and waking activity index) were analyzed using the data averaged over 3 days of LD. Error bars show S.E.M. in each figure. Bars with the same letter indicate values that are not significantly different (P > 0.05). (a) Daily sleep patterns of control and experimental flies. (b) Total sleep amount during day and night. (c) Sleep-bout durations during day and night. (d) Wake-bout durations during day and night. (e) Waking activity indices during day and night. (f) Waking times in the morning (ZT0–4), midday (ZT4–8), and evening (ZT8–12).