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. 2016 Nov 17;6:37255. doi: 10.1038/srep37255

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Sleep/wake parameters were analyzed using the data averaged over 3 days of LD. Error bars show S.E.M. in each figure. Bars with the same letter indicate values that are not significantly different (P > 0.05). (a) Schematic representation of wild-type and truncated forms of AP. (b and c) Possible model of dysfunction of Ap/Chi through expression of Ap^ΔLIM and Ap^ΔHD. Sleep/wake parameters obtained using (d–f) Pdf-GAL4/UAS-ap^ΔHD flies, (g–i) Pdf-GAL4/UAS-ap^ΔLIM flies, and (j–l) Pdf-GAL4/UAS-Chi RNAi flies. (d,g and j) Daily sleep patterns of control and experimental flies. (e,h and k) Total sleep amount during day and night. (f, i and l) Waking times in the morning (ZT0–4), midday (ZT4–8), and evening (ZT8–12).