Figure 1. Protein-free diet during lactation modulates the offspring hepatic CYPs mRNA expression at adulthood. A, representative images of hepatic CYP1A2, CYP2B1, CYP2B2 and CYP2E1 mRNA expression in 15-, 30-, 60- and 90-day-old offspring from dams fed either a 0% protein (MPR groups) or a standard diet (C groups) during the first ten days of lactation, evaluated by RT-PCR in a 6% polyacrylamide gel; CYP1A1 expression was not detected. B, Densitometric analysis of CYP1A2, CYP2B1, CYP2B2 and CYP2E1 mRNA expression. Data are reported as median and standard deviation of three biological replicates and normalized by GAPDH analysis (mRNA expression data derived from three independent pools of 6 males from each group, i.e., N=3). MWM: molecular weight marker (pEJ3 plasmid DNA digested with using Eco147II and PvuI). *P<0.05 for MPR samples compared to their C counterpart samples (Student's t-test).