Figure 2. Maternal protein-free diet during lactation alters the offspring hepatic CYP2B1/2B2 protein expression levels at adulthood. A, representative images of hepatic CYP1A1, CYP1A2, CYP2B1, CYP2B2 and CYP2E1 protein expression in 15-, 30-, 60- and 90-day-old offspring from dams fed either a 0% protein (MPR groups) or a standard diet (C groups) during the first 10 days of lactation, evaluated by western blotting. B, Densitometric analysis of CYP1A1/1A2, CYP2B1/2B2 and CYP2E1 protein expression. Data are reported as median and standard deviation of three biological replicates (protein expression data derived from three independent pools of 6 male rats from each group, i.e., N=3). MWM: molecular weight marker (containing serum bovine albumin and ovalbumin). *P<0.05 for MPR samples compared to their C counterpart samples (Student's t-test).