Figure 6. Unrooted phylogenetic tree of WRKY TFs of dicot and lower eukaryotic (amoeba, algae, bryophyte, pteridophyte, and gymnosperm) plants.

The phylogenetic tree shows the presence of three phylogenetically distinct groups. We named them as groups I (pink), IIa (red), IIb (lime), IIc (blue), and III (green). The WRKY TF group members of group IIa, IIb and IIc overlap with each other and were hence retained under sub-group of group II. The classification of groups I, II, and III resembled that used in previous studies. The WRKY TF members of groups I and III did not overlap with one another and resembled the grouping system of used in previously published studies. The phylogenetic tree was constructed by using MEGA6.