Table 9. Gene duplication analysis of novel WRKY TFs identified during this study.

Figure 1	Genes	Z-score	Percentage of confidence Level
A	GrWRKY12	8.12	100
B	AcWRKY1	6.7	100
C	LuWRKY3-5	4.42	100
D	BrWRKY36-2	8.22	100
E	FvWRKY59	20.58	100
F	PhWRKY59	19.82	100
G	BrWRKY58-1	25.80	100
H	AtWRKY19	25.98	100
I	OsWRKY57	8.43	100
J	FvWRKY52	15.34	100
K	FvWRKY70-7	18.80	100
L	FvWRKY16	6.67	100
M	SbWRKY59	1.78	92.5
N	AlWRKY16	12.53	100
O	PvWRKY94-1	1.11	73.2
P	SiWRKY59-2	1.11	73.4

The result showed that SbWRKY29, PvWRKY94-1 and SiWRKY59-2 are non duplicated WRKY TFs. A z-score value above four is considered duplicated, whereas a value below four was considered nonduplicated. The duplication analysis was performed as described in Pinda (pipeline for intraspecies duplication analysis)⁸¹.