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. 2016 Nov 17;6:37201. doi: 10.1038/srep37201

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Copyright © 2016, The Author(s)

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(a) Genomic identification (P1 single primer extension, upper panel) performed on MNC cells in a representative cohort of n = 6 healthy donors and n = 12 AML patients. In the lower panel the WNT10B and WNT10B^IVS1 gene expression analysis with the GAPDH as housekeeping gene. (b) Schematic diagram of genomic WNT10B locus, consisting in five exons (upper panel). In the lower panel is represented a scheme of the rearrangement involving the WNT10B locus upstream the exon 2, defining the sequence at WNT10B 5′-end flanking region. The electropherogram shows the WNT10B^R sequence and the red line squared non-human sequence extending upstream the portion of IVS1 (AML#9).