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. 2016 Nov 17;6:37225. doi: 10.1038/srep37225

Figure 1. Neutralizing Abs can mediate efficient ADCC against T cells infected with different HIV isolates.

Figure 1

(a–e) CEM CD4+ T cells were infected with prototypic CCR5-tropic NL4.3.ADA.IRES.GFP wild-type (WT) or (d and e) the indicated T/F viruses and analyzed by flow cytometry for Env expression (a and d) and susceptibility to bioluminescence-based ADCC (b, c and e) using indicated anti-HIV NAbs (in μg/ml). (a) Extent of Env staining (in median fluorescence intensity (MFI) units) on GFP+ T cells (n = 3 to 4). (b, c and e) PBMCs from healthy donors were used as effector cells. Percentages of Ab-specific ADCC were determined following subtraction of cell lysis mediated by control IgG from that by test antibody (5 μg/ml). (b) ADCC responses by two donors as examples and (c) Compilation of median ADCC activity from at least 8 different donors. (d) Median Env staining on p24+ T cells from 3 experiments. (e) Boxes and whiskers graph showing median ADCC response from 4 donors. MPER, membrane proximal external region.