Figure 7. Exogenous IFNα treatment and inactivation of Nef and Vpu enhance ADCC activity against HIV latent cells.

Jurkat-based T cells latently infected with CCR5-tropic NL4.3.ADA.IRES.GFP WT or ∆N∆U (N-U-) virus were treated with RMD for 9 h and then with or without IFNα for 15 h. Latent cells were examined by flow cytometry for reappearance of (a) GFP, (b) Env expression and (c and d) ADCC susceptibility using PGT121. Panel c indicates ADCC response from 2 donors as examples and Panel d shows averaged Ab-specific ADCC (±s.d.) from 6–7 donors. Each dot represents 1 donor. In Panels b and d, Mann-Whitney U-tests were used to compare ranks between RMD and RMD + IFN-treated groups with each virus.