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. 2016 Nov 17;6:37183. doi: 10.1038/srep37183

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Copyright © 2016, The Author(s)

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(A) A wound-healing assay showed that scratch wounds closed more slowly in cells transfected with pEGFP-SPRY4-IT1 compared with controls, whereas they closed more quickly in cells transfected with si-SPRY4-IT1. (B) The percentage of the wound closure from the wound-healing assay. (C) The invasion capacity of the cells after knockdown and overexpression of SPRY4-IT1 as determined by Transwell assays (Values are mean ± SD; *P < 0.05; **P < 0.01). (D) The columns indicate the average number of cells from five fields. (E) SPRY4-IT1-silenced cells exhibited a more fibroblast-like shape. Cell morphology of silenced-SPRY4-IT1 HTR-8/SVeno cells and control cells Magnification, 400×; scale bars, 40 μm.