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. 2016 Nov 17;6:37183. doi: 10.1038/srep37183

Figure 4. Changes in EMT markers after overexpression or knockdown of SPRY4-IT1 in HTR-8/SVneo cells.

Figure 4

(A) Western blot assay of E-cadherin, β-catenin, and vimentin expression after SPRY4-IT1 was overexpressed or knocked down in HTR-8/SVneo cells. GAPDH protein was used as an internal control. (B,C) The columns indicate the relative expression of E-cadherin, β-catenin, and vimentin in HTR-8/SVneo cells in the western blot assay. (Values are means ± SD *P < 0.05; **P < 0.01). (D) The expression of E-cadherin, β-catenin, and vimentin detected by qRT- PCR after SPRY4-IT1 was knocked down in cells transfected with si-NC or si-SPRY4-IT1 or overexpressed in cells transfected with pEGFP-SPRY4-IT1 (E).