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. 2016 Nov 17;6:37447. doi: 10.1038/srep37447

Figure 3. The swimming motility of S. Typhimurium was inhibited by blocking QseC.

Figure 3

(A) Expression of the mobility-related gene flhDC was evaluated. RNA was extracted from an overnight culture grown aerobically in LB medium in the absence or presence of Br-LED209. Real-time reverse transcription (RT)-PCR was performed. The rpoA gene was used as the endogenous control. Data were normalized to levels of rpoA and were calculated as fold changes compared to the WT group. (***P < 0.001 vs. WT in one-way ANOVA, n = 3) (B,C) Swimming motility assay. About 1 μl of WT bacteria or the qseC mutant was spotted onto agar plates with or without 200 μM Br-LED209, and halo sizes were measured at 6, 12 and 18 hours after incubation at 37 °C. (*P < 0.05, ***P < 0.001 vs. WT; #P < 0.05 vs. WT+Br-LED209 in two-way ANOVA, n = 3).