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. 2016 Oct 11;36(6):3131–3138. doi: 10.3892/or.2016.5159

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Copyright: © Sun et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Hierarchical clustering analysis and validation of differentially expressed proteins. (A) Hierarchical clustering analysis of 210 dysregulated proteins in RCC compared with the normal renal kidney tissues. (B) Hierarchical clustering analysis of the top 35 up- and downregulated proteins in RCC compared with the normal renal kidney tissues. (C) Validation of PCK1 and SNRPF expression using western blotting. Representative blots showing the expression of PCK1 and SNRPF in four RCC tumor tissues and matched normal kidney tissues. GAPDH was used as a loading control. (D) Graphical representation of the log₂FC in the expression of the two proteins between four RCC tumor tissues and matched normal kidney tissue as determined by densitometry. RCC, renal cell carcinoma; FC, fold change.