Fig. 1.

IFNγ and PD1 methylation in CMV seropositive and CMV seronegative healthy kidney donors. The percentage of DNA methylation of IFNγ (a) and of PD1 (b) in CMV seronegative (n = 15; open bars) and CMV seropositive healthy donors (n = 15; gray bars) in PBMCs (mean ± SD) and stratified by gender (box and whiskers min to max). Gating strategy of the different CD8+ memory T cell subsets in (c). The percentage of DNA methylation of IFNγ (d) and of PD1 (e) in CMV seropositive (n = 5; open bars) and CMV seronegative healthy donors (n = 5; gray bars) in cell sorted CD8+ T cell subsets; naïve, CD27+ memory, CD27− memory, and differentiated effector memory CD45RA+ (EMRA). Box and whiskers (min to max); *p < 0.05 and **p < 0.01