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IFNγ and PD1 protein expression and IFNγ and PD1 DNA methylation. FACS plots of IFNγ and PD1 expression in naïve, CD27+ memory, CD27− memory, and differentiated effector memory CD45RA+ (EMRA) CD8+ T cells in (a; representative example). Mean protein expression and percentage of DNA methylation in the different CD8+ T cell subsets in kidney transplant recipients before transplantation (n = 10; IFNγ in b–d and PD1 in e–g; mean ± SD). ***p < 0.001
