Table 2.

Primers for PCR amplification and pyrosequencing

Gene	Primers	CpGs
IFNγ	F: 5′-ATGGTATAGGTGGGTATAATGG-3′
	R: 5′-biotin-CAATATACTACACCTCCTCTAACTAC-3′
	S: 5′-GGTGGGTATAATGGG-3′	CpG-186
	S: 5′-ATTATTTTATTTTAAAAAATTTGTG-3′	CpG-54
PD1	F: 5′-AGTATAGAATATAAGGAGATAAGTAAGT-3′
	R: 5′-biotin-CCATAACCACAATTCCAAATCTTT-3′
	S: 5′-AGAATATAAGGAGATAAGTAAGTT’-3′	CpG-914, CpG-911, CpG-906
	S: 5′-GGATTTTTTGAATTATTTTATTTTG′-3′	CpG-857, CpG-833
	S: 5′-TTAGTTTTATAGTTAGTTTTTG-3′	CpG-776, CpG-762, CpG-738

F forward primer, R reverse primer, S sequencing primer, CpGs cytosine phosphate guanine sites