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. Author manuscript; available in PMC: 2016 Nov 17.
Published in final edited form as: ACS Chem Neurosci. 2015 Apr 15;6(5):751–760. doi: 10.1021/acschemneuro.5b00013

Figure 4. Filter trap assay of tau filament disassembly.

Figure 4

Tau polymerization reactions were performed with 2 µM tau and 75 µM arachidonic acid at room temperature. After 6 hours, 200 µM compound or an equal volume of DMSO was added to the reactions. The compounds used are listed on the X-axis. The resulting amount of tau filament formation was determined using a filter trap assay. The values for tau filament formation were normalized to the amount of aggregates detected in the absence of compound (dashed line). Negative values indicate that there was less detectable tau on the filter after treatment with a compound than was observed with monomeric tau in the absence of arachidonic acid. The amount of tau on the filter was detected using a mixture of antibodies tau 5, tau 7 and tau 12. Values are the average of three trials ± SD. *P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001