Fig 4. nrg1^nc28 survive to adulthood without overt cardiac abnormalities.

(A-C) Representative gross morphology of age-matched (A) nrg1^WT/WT, (B) nrg1^WT/nc28 or (C) nrg1^nc28/nc28 clutch mates, Standard Length (SL) 25–30. Scale bar is 10mm. (D) Weekly survival of fish from sibling nrg1^WT/nc28 and nrg1^nc28/nc28. (E-F) Representative cross section of the heart in H&E stained section of formaldehyde-fixed, paraffin embedded (E) nrg1^WT/WT and (F) nrg1^nc28/nc28adult fish. BA = Bulbous Arteriosus, V = Ventricle, A = Atrium. N = 3 fish per genotype. Scale bar 100 μm.