Skip to main content
. 2016 Nov 15;11(11):e0166147. doi: 10.1371/journal.pone.0166147

Fig 1. Expression of CRKL and SLC7A5 in cell lines.

Fig 1

(A) Analysis of transcription level of CRKL in cell lines by RT-PCR. The mRNA levels of CRKL in three cell lines (MKN-45, SGC-7901 and SUN-16) was significantly higher than that in GES-1 cells (**P<0.01) (B) Detection of protein expression of CRKL in cell lines by western-blot analysis. CRKL was significantly over-expressed in GC cells compared with GES-1 cells. (C) RT-PCR was conducted to measure the mRNA levels of SLC7A5 in these three cell lines. SLC7A5 mRNA level was significantly higher than that in GES-1 cells (**P<0.01) (D) Western-blot analysis was carried out to measure the protein expression of CRKL. CRKL was significantly over-expressed in GC cells compared with GES-1 cells. The numbers above the blot indicate the normalized protein amounts relative to the negative control, as determined by densitometry. As histograms above shown, SGC-7901 cells presented a highest expression of both CRKL and SLC7A5 among the GC cell lines.