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. 2016 Nov 16;11(11):e0166450. doi: 10.1371/journal.pone.0166450

Fig 1. DRB modulates the phosphorylation and subcellular localization of αB-crystallin.

Fig 1

(A) αB-crystallin knockdown rendered rat articular chondrocytes vulnerable to subtoxic doses of DRB. 24 h after DRB treatment. (B) Knockdown of both αB-crystallin and CK2 genes reduced the viability of rat articular chondrocytes. (C) Overexpression of αB-crystallin reversed the reduced viability of rat articular chondrocytes to toxic doses of DRB. 72 h after DRB treatment. (D) DRB reduced αB-crystallin protein expression and P-αB-crystallin-Ser-19, Ser-45 and Ser-59 levels. Each experiment, six in total, was conducted with three animals each. In each experiment, the cells from three animals were pooled and analyzed in triplicate. Values are the mean ± SD. * P < 0.05, ** P <0.01 vs. control.