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. 2016 Nov 15;11(11):e0165572. doi: 10.1371/journal.pone.0165572

Table 1. Protein Estimation and activity profile at each step of purification.

Step Activity (U/ml) Protein Conc. Amount (ml) Total Protein (mg) Total Activity (U) Specific Activity (U/mg) Fold Purification Yield (%)
Homogenate 30229.746 1.061 150 159.15 4534461.9 189.945 1 100
30–50% (NH4)2SO4 precipitate 164449.82 2.095 26 54.47 4275695.3 3019.0898 15.894 94.293
FPLC eluent 301088.27 0.708 14 9.912 4215235.8 30376.137 159.92 92.96