Table 1. Protein Estimation and activity profile at each step of purification.
Step | Activity (U/ml) | Protein Conc. | Amount (ml) | Total Protein (mg) | Total Activity (U) | Specific Activity (U/mg) | Fold Purification | Yield (%) |
---|---|---|---|---|---|---|---|---|
Homogenate | 30229.746 | 1.061 | 150 | 159.15 | 4534461.9 | 189.945 | 1 | 100 |
30–50% (NH4)2SO4 precipitate | 164449.82 | 2.095 | 26 | 54.47 | 4275695.3 | 3019.0898 | 15.894 | 94.293 |
FPLC eluent | 301088.27 | 0.708 | 14 | 9.912 | 4215235.8 | 30376.137 | 159.92 | 92.96 |