Table 1. Selected genes whose transcription is controlled by HHQ, PQS and/or PqsE.
| PA number a | Gene name a | HHQ b | PQS c | PqsE d | Product name a |
|---|---|---|---|---|---|
| PA0051 | phzH | 3.2 (3.6) | Potential phenazine-modifying enzyme | ||
| PA0083 | tssB1 | -5.1(-1.4) | TssB1 | ||
| PA0084 | tssC1 | -3.5 (-1.3) | TssC1 | ||
| PA0263 | hcpC | -4.3 | Secreted protein Hcp | ||
| PA0997* ∫ ‡ ◊ | pqsB | 6.7 | 17.5 | PqsB | |
| PA0998* ∫ ‡ ◊ | pqsC | 5.5 | 16.1 | PqsC | |
| PA0999* ∫ ‡ ◊ | pqsD | 5.8 | 15.7 | 3-oxoacyl-[acyl-carrier-protein] synthase III | |
| PA1000* ∫ ◊ | pqsE | 22.8 (140.3) | Quinolone signal response protein | ||
| PA1001* ∫ ◊ | phnA | 26.2 (ncd) | Anthranilate synthase component I | ||
| PA1002* ∫ ◊ | phnB | 22.4 (ncd) | Anthranilate synthase component II | ||
| PA1245 § ∫ | aprX | 3.9 | AprX | ||
| PA1706 | pcrV | 2.7 | Type III secretion protein PcrV | ||
| PA1707 | pcrH | 3.1 | Regulatory protein PcrH | ||
| PA1708 | popB | 5.6 | Translocator protein PopB | ||
| PA1709 | popD | 3.0 | Translocator outer membrane protein PopD precursor | ||
| PA1710 | exsC | 3.5 | ExsC exoenzyme S synthesis protein C precursor | ||
| PA1711 | exsE | 3.1 | ExsE | ||
| PA1712 | exsB | 2.6 | Exoenzyme S synthesis protein B | ||
| PA1718 | pscE | 4.3 | Type III export protein PscE | ||
| PA1901 ∫ | phzC2 | 5.5 (6.3) | Phenazine biosynthesis protein PhzC | ||
| PA1902 § | phzD2 | 7.5 (9.8) | Phenazine biosynthesis protein PhzD | ||
| PA1903 ∫ | phzE2 | 8.8 (9.6) | Phenazine biosynthesis protein PhzE | ||
| PA1904 | phzF2 | 10.3 (9.9) | Probable phenazine biosynthesis protein | ||
| PA1905 | phzG2 | 9.7 (9.6) | Probable pyridoxamine 5'-phosphate oxidase | ||
| PA2193* | hcnA | 3.6 (2.1) | Hydrogen cyanide synthase HcnA | ||
| PA2194* | hcnB | 3.1 (1.7) | Hydrogen cyanide synthase HcnB | ||
| PA2195* | hcnC | 3.0 (1.6) | Hydrogen cyanide synthase HcnC | ||
| PA2300* ∫ ◊ | chiC | 18.7 (8.2) | Chitinase | ||
| PA2426 | pvdS | 43.9 | Sigma factor PvdS | ||
| PA2570* ∫ ◊ | lecA | 26.3 (15.1) | LecA lectin | ||
| PA3361* ◊ | lecB | 8.5 (10.4) | Fucose-binding lectin LecB | ||
| PA3391 | nosR | -4.6 | -4.1 | Regulatory protein NosR | |
| PA3478* | rhlB | 3.6 (2.3) | Rhamnosyltransferase chain B | ||
| PA3479 | rhlA | 3.6 (2.3) | Rhamnosyltransferase chain A | ||
| PA3841 | exoS | 2.6 | Exoenzyme S | ||
| PA3842 | spcS | 3.9 | Specific Pseudomonas chaperone for ExoS, SpcS | ||
| PA4175 | prpL | 3.7 | PrpL, protease IV | ||
| PA4205* ∫ ◊ | mexG | 25.0 (47.2) | Hypothetical protein | ||
| PA4206* ∫ ◊ | mexH | 16.4 (28.8) | Probable RND efflux membrane fusion protein precursor | ||
| PA4207* ∫ ◊ | mexI | 18.5 (18.2) | Probable RND efflux transporter | ||
| PA4208* ∫ ◊ | opmD | 11.6 (9.3) | Probable outer membrane protein precursor | ||
| PA4209* ∫ ◊ | phzM | 4.1 (8.7) | Probable phenazine-specific methyltransferase | ||
| PA4210 ◊ | phzA1 | 10.2 (16.2) | Probable phenazine biosynthesis protein | ||
| PA4211* ◊ | phzB1 | 5.6 (10.1) | Probable phenazine biosynthesis protein | ||
| PA4217* § ◊ | phzS | 9.0 (9.3) | Flavin-containing monooxygenase | ||
| PA4227 ∫ ‡ | pchR | 11.2 | Transcriptional regulator PchR | ||
| PA4468 § | sodA | 89.4 | Superoxide dismutase | ||
| PA4470 § ∫ | fumC1 | 114.7 | Fumarate hydratase | ||
| PA4648 | cupE1 | 3.0 (2.2) | Pilin subunit CupE1 |
a PA number, gene name and product name are from the Pseudomonas Genome Database [13]. Genes previously reported as controlled by iron-starvation are in bold characters [40,41].
*, genes whose transcription was altered in the ∆pqsR mutant with respect to the wild type strain [10]
§, genes whose transcription was altered upon exogenous PQS provision [28]
∫, genes whose transcription was altered in the ∆pqsA mutant with respect to the wild type strain [11]
‡, genes whose transcription was altered upon PqsE overexpression [11]
◊, genes whose transcription was altered in the ∆pqsH mutant with respect to the wild type strain [23]. RND, Resistance-Nodulation-Cell division; MFS, major facilitator superfamily.
b Fold change in gene expression in P. aeruginosa PAO1 ∆4AQ grown in the LB supplemented with 40 μM HHQ with respect to the same strain grown in LB.
c Fold change in gene expression in P. aeruginosa PAO1 ∆4AQ grown in the LB supplemented with 40 μM PQS with respect to the same strain grown in LB.
d Fold change in gene expression in P. aeruginosa PAO1 ∆4AQ grown in the LB supplemented with 1 mM IPTG (to induce PqsE expression) with respect to the same strain grown in LB; in brackets is indicates the fold change in gene expression in P. aeruginosa PAO1 ∆pqsAHLE pUCPpqsE with respect to P. aeruginosa PAO1 ∆pqsAHLE pUCP18, both grown in LB. For the microarray analysis performed in the ∆pqsAHLE background, fold changes with a q value < 0.05 are indicated for selected virulence related genes, irrespective of the fold change. ncd, no change detected (q value > 0.05).