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. 2016 Nov 16;11(11):e0166500. doi: 10.1371/journal.pone.0166500

Fig 4. MSC increase the proliferation of HOS-CSC.

Fig 4

Homotypic cultures of CSC or co-cultures of CSC+MSC for 3 days were trypsinized, fixed and immunostained with anti-Ki67 antibody (green). Hoecst 33342(blue) was used to counterstain nuclei. (A) Representative image; (B) The percentage of Ki67 positive nuclei was quantified and expressed as ratio to the total number of cells (*p<0.05). Ki67 expression was increased in the presence of MSC as compared to CSC cultured alone. Images were acquired using the same exposure setting. Original magnification 20X. Figure shows representative images. (C) The number of colonies formed by HOS-CSC co-cultured with MSC were evaluated on soft-agar and quantified. MSC secreatome enhanced HOC-CSC tumorigenicity.