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. 2016 Nov 16;11(11):e0166500. doi: 10.1371/journal.pone.0166500

Fig 5. MSC secrete pro-inflammatory cytokines when exposed to OS CSC whereas CSC are unaffected.

Fig 5

(A) Scheme of the experiments used to evaluate the secreted proteins showed in the following panels; (B) TGF- β1 and IL-6 ELISA assays of the supernatants collected from the different chambers of the transwell with MSC co-cultured with HOS-CSC. TGFβ1 is secreted exclusively by MSC co-coltured in the presence of stem-like cells whereaseIL-6 is secreated by MSC independently from the co-cultuing with MSC. However secreated IL-6 levels were increased in MSC when co-cultured with CSC. The amount of IL-6 detected in lanes 2 and 4 is most likely due to leakage from the upper chamber, as no amount could be detected in the control in lane 6, where no MSC were seeded; (C) IL-6 ELISA assay for the evaluation of the same phenomenon with MG63-CSC secretion. The trend ofMG63-CSC was the same observed for HOS-CSC cells.