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. 2016 Nov 16;11(11):e0166500. doi: 10.1371/journal.pone.0166500

Fig 7. TGFβ1-dependent IL-6 secretion is responsible for increased CSC sphere formation.

Fig 7

(A) HOS-CSC were let to grow for 6 days in the presence of MSC; the latter cells were added every 24 hours with 100 μg/mL of Tocilizumab. Spheres were then photographed, counted, and quantified (scale bar, 500 μm). Representative figures and schematic representation of the performed assay; (B) Treatment with Tocilizumab significantly decreased the number of HOS-CSC spheres showed in panel A (*p<0.05); (C) IL-6 secretion by MSC is TGFβ1 dependent. MSC were treated with 1 μg/mL mAb αTGFβ1 2 hours prior CSC seeding, and re-added every 24 hours for the three days of co-culture. Supernatants from the MSC upper compartment were then collected and analyzed for IL-6 secretion by ELISA assay (*p<0.05).