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. 2016 Nov 15;11(11):e0166660. doi: 10.1371/journal.pone.0166660

Fig 3. Effect of hJagged-1- or hDll-1-expressing HESS-5 stromal cells on the differentiation of CD133+ CB cells.

Fig 3

(A) Flow cytometric analysis of CD31, CD34, CD38, CD133, and KDR expression on CD133+ cells co-cultured with control (C), hJagged-1 (J1)- or hDll-1 (D1)-expressing HESS-5 stromal cells at day 7. Numbers in the graph indicate the percentages of gate events within the quadrant. (B) CD34+/CD38-, CD34+/CD133+, and CD31+/KDR+ cell numbers in 1 × 104 CD133+ cells at 7 days after co-culture with the three types of HESS-5 stromal cells. Data are expressed as means ± SD (n = 5). **P < 0.01, *P < 0.05 between the indicated values. (C) Expanded cell numbers were normalized to the cell numbers activated by control HESS-5 cells at day 7. Data are expressed as means ± SD (n = 5 without GSI; n = 3 with GSI). **P < 0.01, *P < 0.05 between the indicated values. GSI: γ-secretase inhibitor.