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. 2016 Nov 16;11(11):e0164692. doi: 10.1371/journal.pone.0164692

Fig 5. Long-term Stability of Freeze-dried Lead Formulations of H1N1 Subunit Vaccine Stored at 4°C, 25°C, and 37°C.

Fig 5

Two freeze-dried lead formulations were prepared with concentrated bulk H1N1 subunit vaccine (H1N1 California/07/2009 X179A) in phosphate buffer and additional excipients listed in Table 3. The liquid formulation obtained from dilution of bulk vaccine with PBS was used as the control. The stability at 4°C (A), 25°C (B), and 37°C (C) was monitored up to 40 months. For each formulation, three vials were reconstituted and combined. Two dilutions were prepared within the linear range of the standard curve and tested by SRID in triplicate. The data represented the mean HA titer ± standard deviation of six measurements.