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. 2016 Nov 16;11(11):e0164692. doi: 10.1371/journal.pone.0164692

Fig 7. HAI Titers in Mice Immunized with H1N1 (A/Cal/07/2009) Influenza Subunit Vaccine.

Fig 7

Each study group consisted of 10 mice. Animals were immunized intramuscularly with 100 μL of either reconstituted freeze-dried H1N1 subunit vaccine or liquid vaccine diluted to 0.1 μg/mL (upper panel: A) and 1.0 μg/mL (lower panel: B) and blood samples were collected prior to vaccination (weeks 0 and 4) and 4 weeks after the final vaccination (week 8). HAI tiers in serum samples against influenza A/Cal/07/2009 were measured in duplicate, at week 0 (left, pre-immunization), week 4 (middle) and week 8 (right), and were expressed as the reciprocal of the highest dilution demonstrating HA inhibition. Data of individual mice are shown. Geometric mean titer of each group is shown by a horizontal bar. HAI titers induced by immunization at week 4 and week 8 were significantly different from HAI titers at baseline (week 0), (p≤0.002 for groups immunized with 0.01 μg and p<0.05 for groups immunized with 0.1 μg.