Table 2. Composition of Formulations Used in Screening for Freeze-dried H1N1 Subunit Vaccine.
| Formulation | Final Concentration (% w/v) | Final Concentration (mM) | ||||||
|---|---|---|---|---|---|---|---|---|
| ID | Sucrose | Trehalose | Mannitol | Glycine | Dextran 40 | Phosphate | Histidine | |
| Round 1 | Lyo1-F1 | 4 | 20 | |||||
| Lyo1-F2 | 4 | 20 | ||||||
| Lyo1-F3 | 3 | 20 | ||||||
| Lyo1-F4 | 3 | 20 | ||||||
| Lyo1-F5 | 1 | 3 | 20 | |||||
| Lyo1-F6 | 1 | 3 | 20 | |||||
| Lyo1-F7 | 3 | 1 | 20 | |||||
| Round 2 | Lyo2-F1 | 4 | 20 | |||||
| Lyo2-F2 | 1 | 3 | 20 | |||||
| Lyo2-F3 | 3 | 1 | 20 | |||||
| Lyo2-F4 | 4 | 20 | ||||||
| Lyo2-F5 | 1 | 3 | 20 | |||||
| Lyo2-F6 | 3 | 1 | 20 | |||||
| Lyo2-F7 | 4 | 20 | ||||||
| Lyo2-F8 | 1 | 3 | 20 | |||||
| Lyo2-F9 | 3 | 1 | 20 | |||||
The targeted final HA concentration was 45 μg/mL in Round 1 and 90 μg/mL in Round 2. The Round 1 formulations contained 14 mM of NaCl and 0.09% of polysorbate 80, while Round 2 formulations contained 7 mM of NaCl and 0.05% of polysorbate 80. The targeted pH was 7.2.