Fig 9. The effect of MAPK inhibitors on auranofin-induced EGFR/MAPK signaling in ARPE-19 cells.

(A) ARPE-19 cells were pretreated with 1.0 μM SB203580 (P38MAPK inhibitor), 10 μM FR180204 (ERK inhibitor) or 10 μM SP600125 (JNK inhibitor) for 2 hours and further treated with 2.0 μM AF for another 12 hours. Cell lysates were subjected to Western blot analysis. Total and phosphorylated protein level of EGFR, phosphorylated P38MAPK, phosphorylated ERK, phosphorylated JNK, phosphorylated c-Jun, phosphorylated MAPKAPK2 and phosphorylated HSP27 were detected. β-actin was used as a loading control. (B) Quantitation analysis of the results presented in panel A from three independent experiments. * P < 0.05, ** P < 0.01.