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Ratio (CSF fKLC/fLLC)/(Serum fKLC/fLLC) in patients without CSF-restricted o-fLC bands, using the methods (A)–(E). CSF, cerebrospinal fluid; fLC, free light chains; fKLC, free kappa light chains; fLLC, free lambda light chains. (A), Freelite™ assay on the SPAPLUS analyser; (B) N Latex FLC™ assay on BN ProSpec analyser; (C) commercially available ELISA (BioVendor); (D), in-house ELISA using monoclonal standards (Bethyl Laboratories); (E), in-house ELISA using Freelite™ standards.
