Fig 1. DNA barcoding and status of IUCN assessment of ranixalid species of the Western Ghats.

(A) Neighbor-Joining (NJ) tree based on Kimura-2-parameter model for 16S mitochondrial gene sequences from 269 ranixalid samples, representing all previously known and newly recognized species from the Western Ghats. Terminal node labels indicate voucher numbers that are cross-referenced in S2 Table; clade numbers are indicated towards the internal nodes of the tree; mean and range of interspecific genetic distances (in percent) are shown between subsequent clades. (B) Frequency of intra (grey) and interspecific (black) uncorrected pairwise distances for 16S. (C) Number of ranixalid species in the different IUCN categories [66].