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. 2016 Nov 15;11(11):e0166626. doi: 10.1371/journal.pone.0166626

Fig 2. MEK inhibition significantly decreased PD-L1 expression in human lung cancer cell lines.

Fig 2

A, Gating strategy for the determination of PD-L1 levels in lung cancer cell lines stained with FITC anti-PD-L1 mAb. For differentiation between live and dead cells, PI exclusion staining was used. B, (upper) Representative histogram of PD-L1 (shaded histogram) of three KRAS-mutant lung adenocarcinoma cell lines with DMSO or U0126 (20 μM) for 24 h. Empty histogram indicates the isotype control. (upper middle) Relative MFI (PD-L1 MFI / isotype control MFI) of the three cell lines was significantly decreased with U0126. *, p < 0.0001. (lower middle) qRT-PCR also showed a significant decrease of PD-L1 mRNA expression with U0126. *, p < 0.05. Data are the mean of three or four independent experiments. (lower) One representative immunoblot of phospho-ERK.